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Immunophenotyping

The human immune system provides the ideal system for translational human research: Immune cells can be readily isolated from blood and from involved tissues by biopsy. At the core of the Center is a system to perform high-throughput flow cytometry on blood derived cells and then subject isolated cell populations to interrogation. This allows us to perform detailed immunophenotyping of intracellular and cell-surface markers at the level of a population of cells as well as in single cells. To minimize day-to-day variability, we have developed standard operating procedures (SOP) to enable a high-throughput and robust system to process dozens of freshly isolated blood samples each day.

Additional studies include:

CyTOF mass cytometry: Allows simultaneous, quantitative measurements of cellular proteins at the single cell level. Combined staining of cell surface markers and intracellular transcription factors and phosphoproteins will reveal distinct functions and states of activation in individual cells.

Global phosphoproteomics: Phosphoproteomic analyses can performed on purified cell types of moderate cell numbers (approximately 106). Fresh and rapid handling of samples is required. Alternatively, cells can be activated after isolation and subjected to signaling analysis.

Other studies: Beyond immune cell subsets, a variety of other studies can also be arranged on patient samples including serum or urine proteomics and metabolomics, microbiome analysis, tissue biopsy histology etc.

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